![]() ![]() This results in a standardized fluorescence performance so that users do not need to adjust detector voltages.ĭata is collected over 7-decade dynamic range (16 million channels of digital data), making all data available to users as needed. The laser and optical alignments are set and locked down during the manufacturing of the instrument. The Accuri is equipped with two 20mW lasers (488nm and 552nm) and 6 parameters for detection, which include 2 light scatter parameters (forward scatter and side scatter) and 4 parameters for acquiring fluorescence. To schedule Assisted Use or Training please contact Richard Salinas.įees: Trained user: $38/hour. Assisted use: $98/hour BD Accuri SORP Flow Cytometer Instrument Access: Assisted use and training is available. Instrument Configuration: Fortessa Configįluorescence Spectrum Analyzer (BD Biosciences) The Fortessa only accepts 5mL polystyrene tubes, or 96- and 384-multi-well plates if using the HTS unit. Sample analysis can also be done using Flowjo or FCS Express (both available to use in NMS2.114). Sample acquisition and analysis is done using FACSDiva v6.1.3. In addition, this system is also equipped with the FACSFlow Supply System to allow larger volume tanks for sheath and waste, as well as improved fluidic stability. This instrument can be connected to the optional High Throughput Sampler (HTS) unit to accommodate automated specimen handling for 96- and 384-well plates. We’ll just need one of your data files so we can help you set the preferences accordingly.The Fortessa is equipped with four lasers (405nm, 488nm, 561nm and 635nm) and 15 parameters for detection, which includes 2 light scatter parameters (forward and side scatter) and 13 fluorescence parameters. If your data is not scaled properly or you still have trouble, please contact us (techsupport )! We can easily assist you in setting the preferences based on your data. Linear/logarithmic/biexponential default scaling, scale range, and auto-transform can all be set in these cytometer preferences!įor information on getting FlowJo to match the display of acquisition software, choose your vendor below:Īll of these documents are written based on our understanding of the default display of the data on the respective instrument. Each preference set can be adjusted based on how you wish to display the data. To learn more about setting up preferences for the display scaling and transformation of the data from your cytometer, click on the Cytometers button in Preferences. For specific information on data scaling visit here. ![]() Since FlowJo must scale the data identically to what you see on the machine, we have implemented a great new feature in v10 of FlowJo that will create a specific preference set based on the cytometer on which the data was acquired when loaded into FlowJo. However, instruments like the Beckman Coulter Gallios, Life Technologies Attune, BD Accuri C6, and MoFlo Astrios will scale data from 6 to 10 decades. Many people are used to visualizing data in 4 or 5 decades (a scale of 2^10 or 2^18). Instrument scaling range can vary from 2^18 on FACSDiva equipped instruments to 2^32 on MoFlo Astrios. The FCS3.0 standard provides for up to 32 bits of data, or a range in the scale of 2^32. FlowJo can read and analyze FCS files from any cytometer.Įach cytometer will scale your data slightly different during acquisition. As you probably know, FCS (Flow Cytometry Standard) files are created by flow cytometers. ![]()
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